Binding abilities of polyaminocyclodextrins: polarimetric investigations and biological assays

• Marco Russo,
• Daniele La Corte,
• Annalisa Pisciotta,
• Serena Riela,
• Rosa Alduina and
• Paolo Lo Meo

Beilstein J. Org. Chem. 2017, 13, 2751–2763, doi:10.3762/bjoc.13.271

• plasmid DNA. For this, each AmCD was mixed with pDNA at various N/P ratios (average number of nitrogen atoms on the cyclodextrin core/number of phosphate groups of pDNA) and the complexation efficiency was evaluated by electrophoretic mobility shift assays (EMSA). When electrophoresis is applied to pDNA

The effect of cyclodextrin complexation on the solubility and photostability of nerolidol as pure compound and as main constituent of cabreuva essential oil

• Joyce Azzi,
• Pierre-Edouard Danjou,
• David Landy,
• Steven Ruellan,
• Lizette Auezova,
• Hélène Greige-Gerges and
• Sophie Fourmentin

Beilstein J. Org. Chem. 2017, 13, 835–844, doi:10.3762/bjoc.13.84

• -Ner isomer leading to a more stable inclusion complex [16]. An exception is observed for γ-CD, where cis-Ner is better encapsulated than trans-Ner, owing to the larger cavity of this CD. Phase-solubility studies also allow the determination of the complexation efficiency (CE) permitting to evaluate

• in aqueous solution without CD. The solubilizing effect of CD was also determined by calculating complexation efficiency (CE) parameter which is the concentration ratio between CD in a complex and free CD: Total organic carbon (TOC) analysis Phase solubility studies of cabreuva EO with HP-β-CD were

• of 10 mM CD, except for β-CD and γ-CD, 1mM) and complexation efficiency (CE) of cis and trans-Ner with different CDs. Formation constants (Kf) of HP-β-CD inclusion complexes with Ner obtained by phase solubility and UV-visible competition method. 1H NMR chemical shifts (δ, ppm) for free β-CD and β-CD

Determination of formation constants and structural characterization of cyclodextrin inclusion complexes with two phenolic isomers: carvacrol and thymol

• Miriana Kfoury,
• David Landy,
• Steven Ruellan,
• Lizette Auezova,
• Hélène Greige-Gerges and
• Sophie Fourmentin

Beilstein J. Org. Chem. 2016, 12, 29–42, doi:10.3762/bjoc.12.5

• phenomenon in deep. In this study, inclusion complexes were characterized in terms of formation constants (Kf), complexation efficiency (CE), CD:guest molar ratio and increase in bulk formulation by using an UV–visible competitive method, phase solubility studies as well as 1H and DOSY 1H NMR titration

• Phase solubility studies are widely used to evaluate the ability of CDs to increase the aqueous solubility of the guests. They also lead to the determination of diverse parameters involved in complex formation such as Kf value, complexation efficiency (CE), optimal molar ratio for solid inclusion

• slope of the phase solubility profile. The solubilizing capacity of CD was estimated by the complexation efficiency (CE) parameter. CE was calculated from the slope of the phase solubility profile and is equal to the complex to the free CD concentrations ratio: where [CD/guest] is the concentration of

Effects of RAMEA-complexed polyunsaturated fatty acids on the response of human dendritic cells to inflammatory signals

• Éva Rajnavölgyi,
• Renáta Laczik,
• Viktor Kun,
• Lajos Szente and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2014, 10, 3152–3160, doi:10.3762/bjoc.10.332

• lipid rafts in the cell membrane. The complexation efficiency [43] calculated from the solubility data characterize the affinity of the various methylated CDs toward the lipids studied (Table 1). The complex association constants were not calculated because the intrinsic solubility in water is so low

• that it cannot be determined with a precision sufficient for the calculations. The higher complexation efficiency corresponds to a higher affinity for complexation which is manifested in a higher solubilizing effect. Based on these results the inclusion complexes with 1–3% PUFA content were prepared by

• -6 in moDC activated by LPS (A) or by Poly(I:C) (B) (N = 6) (*p < 0.05 compared to the control). The effect of n−3 PUFAs on the expression of GPR120 receptor in resting (red), LPS-activated (yellow) and Poly(I:C)-activated (white) moDC (N = 6) (*p < 0.05 compared to the control). Complexation

Cyclodextrin–polysaccharide-based, in situ-gelled system for ocular antifungal delivery

• Anxo Fernández-Ferreiro,
• Noelia Fernández Bargiela,
• María Santiago Varela,
• Maria Gil Martínez,
• Maria Pardo,
• Antonio Piñeiro Ces,
• José Blanco Méndez,
• Miguel González Barcia,
• Maria Jesus Lamas and
• Francisco.J. Otero-Espinar

Beilstein J. Org. Chem. 2014, 10, 2903–2911, doi:10.3762/bjoc.10.308

• constants (K1:1), complexation efficiency (CE) and the D:CD ratio for each cyclodextrin were calculated and values are listed in Table 1. K1:1 values for the complexes obtained with the two β-CD derivatives were similar to those calculated for the parent β-CD by Upadhyay et al. [16] and for HPBCD by Kutyła

• ) and drug species (guest) in solution. A more accurate method for the determination of the efficiency of the cyclodextrin solubility is to determine their complexation efficiency (CE) [31], that is, the concentration ratio between cyclodextrin in a complex and free cyclodextrin. The CE is calculated

• linear regions obtained by least squares regression using the following equation: The parameter complexation efficiency CE is given by: In addition, the D:CD ratio can be calculated using the CE according to: In order to study the influence of the presence of the two polysaccharides on the complex

Effect of cyclodextrin complexation on phenylpropanoids’ solubility and antioxidant activity

• Miriana Kfoury,
• David Landy,
• Lizette Auezova,
• Hélène Greige-Gerges and
• Sophie Fourmentin

Beilstein J. Org. Chem. 2014, 10, 2322–2331, doi:10.3762/bjoc.10.241

• UV–visible spectroscopy and phase solubility studies. The solubilizing effects of CDs was investigated by determining their complexation efficiency (CE) [18][19]. A theoretical molecular modeling study has been realized to estimate the complexation energies and illustrate the most favorable inclusion

• considerably enhances the PPs solubility (up to 17 fold for 1 with a 10 mM RAMEB solution). Similar findings were found in literature [28][35][36][37][38][39]. The solubilizing effect of CDs can be evaluated using their complexation efficiency (CE), i.e., the concentration ratio between cyclodextrin in a

• potential of CD was evaluated by the complexation efficiency (CE) parameter. CE equal the complex to free CD concentration ratio and was calculated from the slope of the phase solubility diagram [18]: where [CD/PP] is the concentration of dissolved inclusion complex and [CD] is the concentration of

End group functionalization of poly(ethylene glycol) with phenolphthalein: towards star-shaped polymers based on supramolecular interactions

• Carolin Fleischmann,
• Hendrik Wöhlk and
• Helmut Ritter

Beilstein J. Org. Chem. 2014, 10, 2263–2269, doi:10.3762/bjoc.10.235

• polymers requires the complexation of PEG-PP molecules by at least three β-CD moieties attached to the same DPE-CD molecule. We assumed that the complexation efficiency of DPE-CD can be evaluated by comparison of the hydrodynamic diameters of free DPE-CD and the diameter of the complex formed by both